Research Paper Volume 13, Issue 8 pp 12160—12178

Balasubramide derivative 3C attenuates atherosclerosis in apolipoprotein E-deficient mice: role of AMPK-STAT1-STING signaling pathway

Treatment of ApoE-/- mice with 3C inhibits vascular inflammation. (A) Representative images of immunofluorescence staining for ICAM-1 as an indicator of the vascular inflammation from the aortic root of ApoE-/- mice treated with vehicle or 3C. (B) Quantification of ICAM-1 positive areas in plaques (n=6 per group). (C) Representative images of immunofluorescence staining for VCAM-1 form the aortic root of ApoE-/- mice treated with vehicle or 3C. (D) Quantification of VCAM-1 positive areas in plaques (n=6 per group). (E) Protein expression level of ICAM-1 and VCAM-1 protein in the aorta were assessed by Western blot analysis. (F) Quantitative results for the relative expression level of ICAM-1 and VCAM-1 normalized to β-Actin (n=3 per group). All data were assessed using Student’s t-test and are present as mean±SEM. *P **P

Figure 2. Treatment of ApoE-/- mice with 3C inhibits vascular inflammation. (A) Representative images of immunofluorescence staining for ICAM-1 as an indicator of the vascular inflammation from the aortic root of ApoE-/- mice treated with vehicle or 3C. (B) Quantification of ICAM-1 positive areas in plaques (n=6 per group). (C) Representative images of immunofluorescence staining for VCAM-1 form the aortic root of ApoE-/- mice treated with vehicle or 3C. (D) Quantification of VCAM-1 positive areas in plaques (n=6 per group). (E) Protein expression level of ICAM-1 and VCAM-1 protein in the aorta were assessed by Western blot analysis. (F) Quantitative results for the relative expression level of ICAM-1 and VCAM-1 normalized to β-Actin (n=3 per group). All data were assessed using Student’s t-test and are present as mean±SEM. *P <0.05; **P<0.01.