Research Paper Volume 13, Issue 8 pp 12160—12178

Balasubramide derivative 3C attenuates atherosclerosis in apolipoprotein E-deficient mice: role of AMPK-STAT1-STING signaling pathway

Treatment with 3C regulates the HFD induced dysfunctional lipid profile. (A) Weight gain curve of ApoE-/- mice from different groups. (B) Average food intake of every ApoE-/- mouse from different groups. (C) Representative images of paraffin section of the liver staining with H&E staining. (D) Representative images of paraffin section of epididymal fat with H&E staining. (E) Quantitation of relative area of per adipocyte. (F) Quantification of plasma levels of triglycerides, total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in serum from different groups. (G) Fold change of ACC, FASN, SCD-1, HMGCoAs, SREBP-1c, CD36, and PPAR-γ mRNA expression level from livers of different groups (n=6 per group). (H) The OPLS-DA scores plot showing the groupings of 3C (also called P2, green), and vehicle (red) individuals based on their lipid profiles. (I) Clustered heat image map of the relationship between differential lipids and biochemical parameters. All data were assessed using Student’s t-test and are present as mean±SEM. *P**P

Figure 3. Treatment with 3C regulates the HFD induced dysfunctional lipid profile. (A) Weight gain curve of ApoE-/- mice from different groups. (B) Average food intake of every ApoE-/- mouse from different groups. (C) Representative images of paraffin section of the liver staining with H&E staining. (D) Representative images of paraffin section of epididymal fat with H&E staining. (E) Quantitation of relative area of per adipocyte. (F) Quantification of plasma levels of triglycerides, total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in serum from different groups. (G) Fold change of ACC, FASN, SCD-1, HMGCoAs, SREBP-1c, CD36, and PPAR-γ mRNA expression level from livers of different groups (n=6 per group). (H) The OPLS-DA scores plot showing the groupings of 3C (also called P2, green), and vehicle (red) individuals based on their lipid profiles. (I) Clustered heat image map of the relationship between differential lipids and biochemical parameters. All data were assessed using Student’s t-test and are present as mean±SEM. *P<0.05; **P<0.01.