Research Paper Volume 13, Issue 8 pp 12160—12178

Balasubramide derivative 3C attenuates atherosclerosis in apolipoprotein E-deficient mice: role of AMPK-STAT1-STING signaling pathway

Treatment of ApoE-/- mice with 3C inhibits JAK-STAT1 and downstream signaling. (A) Relative level of P-Stat1; Stat1; P-AMPK; AMPK; STING; P-IRF3 and IRF3 was assessed by Western Blot (n=3 per group). (B) Quantitative results for the relative expression level of P-Stat1; P-AMPK; STING and P-IRF3normalized to β-Actin (n=3 per group). (C) Fold change of STING, IFN-α, IFN-β, and CXCL10 mRNA expression level from aortas of different groups. (D) Representative images of immunofluorescence staining for Stat1 from the aortic root of ApoE-/- mice treated with vehicle or 3C. All data were assessed using Student’s t-test and are present as mean±SEM. *P

Figure 8. Treatment of ApoE-/- mice with 3C inhibits JAK-STAT1 and downstream signaling. (A) Relative level of P-Stat1; Stat1; P-AMPK; AMPK; STING; P-IRF3 and IRF3 was assessed by Western Blot (n=3 per group). (B) Quantitative results for the relative expression level of P-Stat1; P-AMPK; STING and P-IRF3normalized to β-Actin (n=3 per group). (C) Fold change of STING, IFN-α, IFN-β, and CXCL10 mRNA expression level from aortas of different groups. (D) Representative images of immunofluorescence staining for Stat1 from the aortic root of ApoE-/- mice treated with vehicle or 3C. All data were assessed using Student’s t-test and are present as mean±SEM. *P<0.05.