Research Paper Volume 13, Issue 9 pp 12780—12799

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis


Figure 3. CircRNA_30032 silencing attenuates TGF-β1-induced expression of collagen I, collagen III and fibronectin in BUMPT cells. (A) RT-qPCR analysis shows the circRNA_30032 expression levels in BUMPT cells transfected with 50 nM circRNA_30032 siRNA or scrambled siRNA, and then treated with or without 5ng/mL TGF-β1 for 24 h. (B) Representative western blot images and (CE) densitometric estimation of (B, C) collagen I, (B, D) collagen III, and (B, E) fibronectin protein levels in control and circRNA_30032-silenced BUMPT cells treated with or without 5ng/mL TGF-β1 for 24 h. Note: The data are expressed as means ± SD (n = 6). # denotes p<0.05 while comparing scrambled siRNA plus TGF-β1 or circRNA_30032 siRNA plus saline groups vs. scrambled siRNA plus saline group; * denotes p<0.05 while comparing circRNA_30032 siRNA plus TGF-β1group vs. scrambled siRNA plus TGF-β1 group.