Research Paper Volume 13, Issue 10 pp 13726—13738

Long non-coding RNA FEZF1-AS1 promotes the proliferation and metastasis of hepatocellular carcinoma via targeting miR-107/Wnt/β-catenin axis

Downregulation of FEZF1-AS1 inhibited the proliferation and invasion of HCC cells by targeting miR-107/Wnt/β-catenin axis. (A, B) The expression levels of miR-107 and β-catenin mRNA in HCC tissues and adjacent normal tissues were determined using qRT-PCR. (C–F) The relative expression levels of β-catenin, wnt3a and/or p-GSK3β were detected by qRT-PCR and western blot after cell transfection with mimic-NC and mimic-miR-107, *P G, H) Cell proliferation was detected by CCK8 assay, *P #P I–K) Cell apoptosis was detected by flow cytometry, *P #P L–N) Cell invasion was accessed by transwell chamber assay, *P #P

Figure 5. Downregulation of FEZF1-AS1 inhibited the proliferation and invasion of HCC cells by targeting miR-107/Wnt/β-catenin axis. (A, B) The expression levels of miR-107 and β-catenin mRNA in HCC tissues and adjacent normal tissues were determined using qRT-PCR. (CF) The relative expression levels of β-catenin, wnt3a and/or p-GSK3β were detected by qRT-PCR and western blot after cell transfection with mimic-NC and mimic-miR-107, *P < 0.05. SNU-398 and SNU-449 cells were divided into three groups, sh-NC + OE-NC, sh–FEZF1-AS1 + OE-NC, and sh–FEZF1-AS1 + OE-β-catenin and submitted to the following assays. (G, H) Cell proliferation was detected by CCK8 assay, *P <0 .01, sh–FEZF1-AS1 + OE-NC group vs. sh-NC + OE-NC group; #P < 0.05, sh-FEZF1-AS1 + OE-β-catenin group vs. sh–FEZF1-AS1 + OE-NC group. (IK) Cell apoptosis was detected by flow cytometry, *P < 0.01, sh–FEZF1-AS1 + OE-NC group vs. sh-NC + OE-NC group; #P < 0.05, sh-FEZF1-AS1 + OE-β-catenin group vs. sh–FEZF1-AS1 + OE-NC group. (LN) Cell invasion was accessed by transwell chamber assay, *P < 0.01, sh–FEZF1-AS1 + OE-NC group vs. sh-NC + OE-NC group; #P < 0.05, sh-FEZF1-AS1 + OE-β-catenin group vs. sh–FEZF1-AS1 + OE-NC group.