Research Paper Volume 13, Issue 10 pp 13726—13738

Long non-coding RNA FEZF1-AS1 promotes the proliferation and metastasis of hepatocellular carcinoma via targeting miR-107/Wnt/β-catenin axis

Downregulation of FEZF1-AS1 inhibited the migration and EMT of HCC cells by targeting miR-107/Wnt/β-catenin axis. SNU-398 and SNU-449 cells were divided into three groups, sh-NC + OE-NC, sh–FEZF1-AS1 + OE-NC, and sh–FEZF1-AS1 + OE-β-catenin and submitted to the following assays. (A–B) Cell migration was detected by using the wound healing assay, *P #P C–D) The expression of EMT markers (E-cadherin, ICAM1 and Vimentin) were measured by western blot, sh-FEZF1-AS1 group vs sh-NC group, *P #P

Figure 6. Downregulation of FEZF1-AS1 inhibited the migration and EMT of HCC cells by targeting miR-107/Wnt/β-catenin axis. SNU-398 and SNU-449 cells were divided into three groups, sh-NC + OE-NC, sh–FEZF1-AS1 + OE-NC, and sh–FEZF1-AS1 + OE-β-catenin and submitted to the following assays. (AB) Cell migration was detected by using the wound healing assay, *P < 0.01, sh–FEZF1-AS1 + OE-NC group vs. sh-NC + OE-NC group; #P < 0.05, sh-FEZF1-AS1 + OE-β-catenin group vs. sh–FEZF1-AS1 + OE-NC group. (CD) The expression of EMT markers (E-cadherin, ICAM1 and Vimentin) were measured by western blot, sh-FEZF1-AS1 group vs sh-NC group, *P < 0.01, sh–FEZF1-AS1 + OE-NC group vs. sh-NC + OE-NC group; #P < 0.05, sh-FEZF1-AS1 + OE-β-catenin group vs. sh–FEZF1-AS1 + OE-NC group.