Research Paper Volume 13, Issue 10 pp 13788—13806

Redd1 knockdown prevents doxorubicin-induced cardiac senescence

Redd1 suppression attenuates Dox-induced senescence in cardiomyocytes. (A) Representative images of SA-β-gal staining in Dox-challenged H9c2 cardiomyocytes following Redd1 knockdown or overexpression. (B) Quantitative analysis of SA-β-gal activity; the number of SA-β-gal positive cells observed in at least 6 separate microscopic fields is indicated (n = 6 per group). *p &p C–F) Effects of Redd1 overexpression and knockdown on p16INK4a and p21 expression in Dox-challenged H9c2 cardiomyocytes (n = 3 per group). *p &p G–J) Effect of Redd1 overexpression and knockdown on IL-1β, IL-6, IL-12, and TNFα mRNA in Dox-stimulated H9c2 cardiomyocytes (n = 3 per group). Data are mean ± SEM. *p &p

Figure 3. Redd1 suppression attenuates Dox-induced senescence in cardiomyocytes. (A) Representative images of SA-β-gal staining in Dox-challenged H9c2 cardiomyocytes following Redd1 knockdown or overexpression. (B) Quantitative analysis of SA-β-gal activity; the number of SA-β-gal positive cells observed in at least 6 separate microscopic fields is indicated (n = 6 per group). *p < 0.05 vs. the Lv-NC group. &p < 0.05 vs. the Lv-shNC group. (CF) Effects of Redd1 overexpression and knockdown on p16INK4a and p21 expression in Dox-challenged H9c2 cardiomyocytes (n = 3 per group). *p < 0.05 vs. the Lv-NC group. &p < 0.05 vs. the Lv-shNC group. (GJ) Effect of Redd1 overexpression and knockdown on IL-1β, IL-6, IL-12, and TNFα mRNA in Dox-stimulated H9c2 cardiomyocytes (n = 3 per group). Data are mean ± SEM. *p < 0.05 vs. the Lv-NC group. &p < 0.05 vs. the Lv-shNC group.