Figure 4. Azilsartan ameliorated ox-LDL-induced reduction of eNOS and nitric oxide (NO). Cells were stimulated with ox-LDL (100 μg/mL) in the presence or absence of Azilsartan (3, 6 μM) for 24 hours. (A) Gene expression of eNOS; (B) Protein of eNOS; (C) Production of NO. Scale bar, 200 μm (****, P<0.0001 vs. vehicle group; ##, ###, P<0.01, 0.001 vs. ox-LDL group, N=5-6 for each group).