Figure 3. ESCC-secreted exosomal uc.189 promotes lymphangiogenesis. (A) Relative uc.189 levels in EC9706 stably transfected with overexpression and negative control were detected verifiably by qRT-PCR in cells and exosomes. (B) Cellular and exosomal uc.189 levels after transfected with silencing of uc.189 and negative control lentivectors in EC9706 were detected by qRT-PCR. (C–D, upper panel) Migration assay of HLECs was treated previously with indicating exosomes. The average number of migrated cells was calculated under a light microscope. (D, lower panel) Tube formation assay in HLECs pretreated with indicated exosomes are shown. The average length of tubes per field was calculated. (E) CCK-8 proliferation assay in HLECs pre-treated with the indicated exosomes. Error bars represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01.