Research Paper Volume 13, Issue 10 pp 13846—13858

Esophageal carcinoma cell-excreted exosomal uc.189 promotes lymphatic metastasis

Exosomal uc.189 targets EPHA2 inducing lymphangiogenesis in HLECs. (A) DNA binding site sequence between uc.189 and the 3′-UTR of EPHA2 and the sequence of wild type (WT) or mutant type (MT) were shown. (B) The effect of uc.NC and uc.189 on the activity of the luciferase reporter containing either WT or MT were detected by dual-luciferase reporter assay. (C–D) RNA and protein levels of EPHA2 were, respectively, tested by qRT-PCR and western blot in HLECs transfected with uc.189 or negative control (NC) compared with those treated with the indicated exosomes. *P

Figure 4. Exosomal uc.189 targets EPHA2 inducing lymphangiogenesis in HLECs. (A) DNA binding site sequence between uc.189 and the 3′-UTR of EPHA2 and the sequence of wild type (WT) or mutant type (MT) were shown. (B) The effect of uc.NC and uc.189 on the activity of the luciferase reporter containing either WT or MT were detected by dual-luciferase reporter assay. (CD) RNA and protein levels of EPHA2 were, respectively, tested by qRT-PCR and western blot in HLECs transfected with uc.189 or negative control (NC) compared with those treated with the indicated exosomes. *P < 0.05.