Research Paper Volume 13, Issue 10 pp 13846—13858

Esophageal carcinoma cell-excreted exosomal uc.189 promotes lymphatic metastasis

Rescue assays detected the effects of EPHA2 regulated by uc.189 in lymphangiogenesis. (A–B) RNA and protein levels of EPHA2 were detected respectively by qRT-PCR and western blot in HLECs with relative exosomes in the presence of EPHA2 overexpression or vector control and si-EPHA2 or siRNA control. (C–E) Overexpression of EPHA2 rescued the biologic effects via exosomal uc.189-induced, whereas knockdown of EPHA2 simulated the effects associated with exosomal uc.189 through cell migration and tube formation assays in HLECs. Error bars represent the mean ± SD of three independent experiments. *P **P ***P

Figure 5. Rescue assays detected the effects of EPHA2 regulated by uc.189 in lymphangiogenesis. (AB) RNA and protein levels of EPHA2 were detected respectively by qRT-PCR and western blot in HLECs with relative exosomes in the presence of EPHA2 overexpression or vector control and si-EPHA2 or siRNA control. (CE) Overexpression of EPHA2 rescued the biologic effects via exosomal uc.189-induced, whereas knockdown of EPHA2 simulated the effects associated with exosomal uc.189 through cell migration and tube formation assays in HLECs. Error bars represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.