Research Paper Volume 13, Issue 10 pp 13846—13858

Esophageal carcinoma cell-excreted exosomal uc.189 promotes lymphatic metastasis


Figure 5. Rescue assays detected the effects of EPHA2 regulated by uc.189 in lymphangiogenesis. (AB) RNA and protein levels of EPHA2 were detected respectively by qRT-PCR and western blot in HLECs with relative exosomes in the presence of EPHA2 overexpression or vector control and si-EPHA2 or siRNA control. (CE) Overexpression of EPHA2 rescued the biologic effects via exosomal uc.189-induced, whereas knockdown of EPHA2 simulated the effects associated with exosomal uc.189 through cell migration and tube formation assays in HLECs. Error bars represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.