Figure 5. Blocking MELK diminishes the EMT progress in OSCC cell lines. Representative immunoblotting analysis of MELK, E-cad, Slug and vimentin expression after transfection of shMELK in SCC9 (A) and Fadu (B) cell. Immunofluorescence analysis of MELK, vimentin and E-cadherin in SCC9 (C) and FaDu (D) cells transfected with shMELK. Scale bars = 10 μm. *P < 0.05, **P < 0.01.