Research Paper Volume 13, Issue 10 pp 13909—13925

Integrated analysis of long noncoding RNAs and mRNA expression profiles reveals the potential role of lncRNAs in early stage of post-peripheral nerve injury in Sprague-Dawley rats

Construction of lncRNA-mRNA networks. DElncRNAs from top enriched GO terms and KEGG pathways and their target mRNAs were used to construct lncRNA-mRNA networks. In the network, upregulated mRNAs are displayed as red ellipses, downregulated mRNAs are displayed as green ellipses, upregulated lncRNAs are displayed as red rectangles, and downregulated lncRNAs are displayed as green rectangles. Solid lines connect lncRNAs and their cis-acting target genes, and dotted lines connect lncRNAs and their trans-acting target genes. (A) network constructed in GO term wound healing (top enriched GO term of DElncRNAs unique to the control vs D4 group). (B) network constructed in KEGG PI3K-Akt pathway (top enriched KEGG pathway of DElncRNAs unique to the control vs D4 group). (C) network constructed in GO term axongenesis (top enriched GO term of common DElncRNAs). (D) network constructed in KEGG cell cycle pathway (top enriched KEGG pathway of common DElncRNAs). (E) network constructed in GO term ion transportation (top enriched GO term of DElncRNAs unique to the control vs D7 group). (F) network constructed in KEGG hematopoiesis pathway (top enriched KEGG pathway of DElncRNAs unique to the control vs D7 group).

Figure 6. Construction of lncRNA-mRNA networks. DElncRNAs from top enriched GO terms and KEGG pathways and their target mRNAs were used to construct lncRNA-mRNA networks. In the network, upregulated mRNAs are displayed as red ellipses, downregulated mRNAs are displayed as green ellipses, upregulated lncRNAs are displayed as red rectangles, and downregulated lncRNAs are displayed as green rectangles. Solid lines connect lncRNAs and their cis-acting target genes, and dotted lines connect lncRNAs and their trans-acting target genes. (A) network constructed in GO term wound healing (top enriched GO term of DElncRNAs unique to the control vs D4 group). (B) network constructed in KEGG PI3K-Akt pathway (top enriched KEGG pathway of DElncRNAs unique to the control vs D4 group). (C) network constructed in GO term axongenesis (top enriched GO term of common DElncRNAs). (D) network constructed in KEGG cell cycle pathway (top enriched KEGG pathway of common DElncRNAs). (E) network constructed in GO term ion transportation (top enriched GO term of DElncRNAs unique to the control vs D7 group). (F) network constructed in KEGG hematopoiesis pathway (top enriched KEGG pathway of DElncRNAs unique to the control vs D7 group).