Research Paper Volume 13, Issue 9 pp 13087—13107

The expression of mimecan in adrenal tissue plays a role in an organism’s responses to stress

Localization of mimecan expression in mouse adrenal glands using in situ hybridization and immunohistochemistry. (A) Positive staining (red) of sections of adrenal glands hybridized with DIG-labeled mimecan antisense probes primarily appeared in the adrenal cortex and some of the medullary mesenchyme (image obtained under visible light at 10× magnification). (B) The field shown is the same as (A) under fluorescent light. (C) Sections of adrenal glands hybridized with fluorescein-labeled mimecan and DIG-labeled PNMT antisense probes, confirming mimecan expression (red) primarily within the adrenal cortex and PNMT (phenylethanolamine-N methyl transferase; blue) expression in the medulla; no co-expression was observed (image obtained under visible light at 10× magnification). (D) The field shown is the same as (C) under fluorescent light. (E) Sections of adrenal glands hybridized with fluorescein-labeled mimecan and DIG-labeled TH antisense probes, confirming mimecan expression (red) primarily within the adrenal cortex, while TH (blue) was found in the medulla; no co-expression was observed (image obtained under visible light at 10× magnification). (F) The field shown is the same as (E) under fluorescent light. (G) Sections of adrenal glands hybridized with fluorescein-labeled mimecan and DIG-labeled ADREAM antisense probes, confirming mimecan expression (red) primarily within the adrenal cortex. Mimecan and ADREAM co-expression was observed in the medulla (image obtained under visible light). (H) The field shown is the same as (G) under fluorescent light. (I) Sections of formalin-fixed, paraffin-embedded adrenal glands immunostained with rabbit anti-human mimecan antibody and anti-rabbit IgG antibody. Positive (brown) staining was found primarily in the adrenal cortex. (J) Negative control of (I) with pre-immune rabbit serum used in lieu of mimecan antibody. (K–L) Higher magnifications of (I, J).

Figure 1. Localization of mimecan expression in mouse adrenal glands using in situ hybridization and immunohistochemistry. (A) Positive staining (red) of sections of adrenal glands hybridized with DIG-labeled mimecan antisense probes primarily appeared in the adrenal cortex and some of the medullary mesenchyme (image obtained under visible light at 10× magnification). (B) The field shown is the same as (A) under fluorescent light. (C) Sections of adrenal glands hybridized with fluorescein-labeled mimecan and DIG-labeled PNMT antisense probes, confirming mimecan expression (red) primarily within the adrenal cortex and PNMT (phenylethanolamine-N methyl transferase; blue) expression in the medulla; no co-expression was observed (image obtained under visible light at 10× magnification). (D) The field shown is the same as (C) under fluorescent light. (E) Sections of adrenal glands hybridized with fluorescein-labeled mimecan and DIG-labeled TH antisense probes, confirming mimecan expression (red) primarily within the adrenal cortex, while TH (blue) was found in the medulla; no co-expression was observed (image obtained under visible light at 10× magnification). (F) The field shown is the same as (E) under fluorescent light. (G) Sections of adrenal glands hybridized with fluorescein-labeled mimecan and DIG-labeled ADREAM antisense probes, confirming mimecan expression (red) primarily within the adrenal cortex. Mimecan and ADREAM co-expression was observed in the medulla (image obtained under visible light). (H) The field shown is the same as (G) under fluorescent light. (I) Sections of formalin-fixed, paraffin-embedded adrenal glands immunostained with rabbit anti-human mimecan antibody and anti-rabbit IgG antibody. Positive (brown) staining was found primarily in the adrenal cortex. (J) Negative control of (I) with pre-immune rabbit serum used in lieu of mimecan antibody. (KL) Higher magnifications of (I, J).