Research Paper Volume 13, Issue 9 pp 13108—13123

Neutrophil extracellular traps, released from neutrophil, promote microglia inflammation and contribute to poor outcome in subarachnoid hemorrhage

Peripheral blood level of neutrophils is increased in SAH and correlates with functional outcome in patients. (A) Peripheral blood level of neutrophils in patients with SAH within 24 hour compared to that of controls (p t-test). (B) Peripheral blood level of lymphocytes in patients with SAH within 24 hour compared to that of controls (p t-test). (C) Peripheral blood level of monocytes in patients with SAH within 24 hour compared to that of controls (p = 0.272, Student t-test). (D) Peripheral blood level of eosinophils in patients with SAH within 24 hour compared to that of controls (p = 0.105, Mann-Whitney test). (E) Peripheral blood level of basophils in patients with SAH within 24 hour compared to that of controls (n = 20) (p = 0.096, Mann-Whitney test). (F) Initial Hunt and Hess classification and peripheral blood level of neutrophils within 24 hours are positively correlated after SAH (p G) Patients with SAH who developed poor outcome (modified Rankin Scale [mRS] > 2) at 3 months had higher peripheral blood level of neutrophils within 24 hours compared to those who had good outcome (mRS p t-test). (H) There is a positive correlation between mRS-3 months and peripheral blood level of neutrophils within 24 hours (p = 0.008, Spearman correlation). (I) Initial Hunt and Hess classification and peripheral blood level of lymphocytes within 24 hours are not positively correlated after SAH (p = 0.892, Spearman correlation). (J) Patients with SAH who developed poor outcome (modified Rankin Scale [mRS] > 2) at 3 months did not have significant change of peripheral blood level of lymphocytes within 24 hours compared to those who had good outcome (mRS p = 0.306, Student t-test). (K) There is not a positive correlation between mRS-3 months and peripheral blood level of lymphocytes within 24 hours (p = 0.819, Spearman correlation).

Figure 2. Peripheral blood level of neutrophils is increased in SAH and correlates with functional outcome in patients. (A) Peripheral blood level of neutrophils in patients with SAH within 24 hour compared to that of controls (p < 0.001, Student t-test). (B) Peripheral blood level of lymphocytes in patients with SAH within 24 hour compared to that of controls (p < 0.001, Student t-test). (C) Peripheral blood level of monocytes in patients with SAH within 24 hour compared to that of controls (p = 0.272, Student t-test). (D) Peripheral blood level of eosinophils in patients with SAH within 24 hour compared to that of controls (p = 0.105, Mann-Whitney test). (E) Peripheral blood level of basophils in patients with SAH within 24 hour compared to that of controls (n = 20) (p = 0.096, Mann-Whitney test). (F) Initial Hunt and Hess classification and peripheral blood level of neutrophils within 24 hours are positively correlated after SAH (p < 0.001, Spearman correlation). (G) Patients with SAH who developed poor outcome (modified Rankin Scale [mRS] > 2) at 3 months had higher peripheral blood level of neutrophils within 24 hours compared to those who had good outcome (mRS < 2) at 3 months (p < 0.001, Student t-test). (H) There is a positive correlation between mRS-3 months and peripheral blood level of neutrophils within 24 hours (p = 0.008, Spearman correlation). (I) Initial Hunt and Hess classification and peripheral blood level of lymphocytes within 24 hours are not positively correlated after SAH (p = 0.892, Spearman correlation). (J) Patients with SAH who developed poor outcome (modified Rankin Scale [mRS] > 2) at 3 months did not have significant change of peripheral blood level of lymphocytes within 24 hours compared to those who had good outcome (mRS < 2) at 3 months (p = 0.306, Student t-test). (K) There is not a positive correlation between mRS-3 months and peripheral blood level of lymphocytes within 24 hours (p = 0.819, Spearman correlation).