Research Paper Volume 13, Issue 9 pp 13108—13123

Neutrophil extracellular traps, released from neutrophil, promote microglia inflammation and contribute to poor outcome in subarachnoid hemorrhage

The role of NETs on pro-inflammatory subtype transition of BV-2 cells. (A) Study design in vitro and representative pictures of neutrophil from different mice. Scale bar = 10 μm. (B) Representative pictures of TUNEL positive cells in different groups. Scale bar = 50 μm. (C) Representative pictures of CD16/32 positive BV-2 cells in different groups. Scale bar = 50 μm. (D) Representative pictures of CD16/32 positive cells from FACS and Quantitative analysis of MFI of CD16/32. n = 4/group in Con, Neu and Neu+NETs+DNAse I groups, n = 6/group in Neu+NETs group. (E) Representative western blotting images of TNF-α expression in different groups. (F) Quantitative analysis of TNF-α expression in different groups. n = 5/group. (G) Representative western blotting images of IL1β expression in different groups. (H) Quantitative analysis of IL1β expression in different groups. n = 5/group. *P #P

Figure 7. The role of NETs on pro-inflammatory subtype transition of BV-2 cells. (A) Study design in vitro and representative pictures of neutrophil from different mice. Scale bar = 10 μm. (B) Representative pictures of TUNEL positive cells in different groups. Scale bar = 50 μm. (C) Representative pictures of CD16/32 positive BV-2 cells in different groups. Scale bar = 50 μm. (D) Representative pictures of CD16/32 positive cells from FACS and Quantitative analysis of MFI of CD16/32. n = 4/group in Con, Neu and Neu+NETs+DNAse I groups, n = 6/group in Neu+NETs group. (E) Representative western blotting images of TNF-α expression in different groups. (F) Quantitative analysis of TNF-α expression in different groups. n = 5/group. (G) Representative western blotting images of IL1β expression in different groups. (H) Quantitative analysis of IL1β expression in different groups. n = 5/group. *P < 0.05 versus Neu, #P < 0.05 versus Neu+NETs group.