Research Paper Volume 13, Issue 11 pp 14892—14909

Inhibition of CDK9 attenuates atherosclerosis by inhibiting inflammation and phenotypic switching of vascular smooth muscle cells

CDK9 is expressed in VSMCs in atherosclerotic lesions. (A) Serum levels of CDK9 were detected using ELISA assay (n = 8; **P B) p-CDK9 and CDK9 protein levels in aortas of ApoE-/- mice were detected by western blotting. (C) Representative immunofluorescence staining of CDK9 (red) and macrophage marker CD68 (green). Tissues were counterstained with DAPI (blue). Yellow arrows indicate co-location of CDK9 and CD68 stanning (scale bar = 50 μm). (D) Representative immunofluorescence staining of CDK9 (red) and VSMCs marker α-SMA (green). Tissues were counterstained with DAPI (blue). Yellow arrows indicate co-location of CDK9 and α-SMA stanning (scale bar = 50 μm). (E, F) Western blot analysis of p-CDK9 and CDK9 protein levels in VSMCs challenged with 50 μg/ml ox-LDL for the indicated time points (n = 8; ##P

Figure 1. CDK9 is expressed in VSMCs in atherosclerotic lesions. (A) Serum levels of CDK9 were detected using ELISA assay (n = 8; **P < 0.01 compared to STD). (B) p-CDK9 and CDK9 protein levels in aortas of ApoE-/- mice were detected by western blotting. (C) Representative immunofluorescence staining of CDK9 (red) and macrophage marker CD68 (green). Tissues were counterstained with DAPI (blue). Yellow arrows indicate co-location of CDK9 and CD68 stanning (scale bar = 50 μm). (D) Representative immunofluorescence staining of CDK9 (red) and VSMCs marker α-SMA (green). Tissues were counterstained with DAPI (blue). Yellow arrows indicate co-location of CDK9 and α-SMA stanning (scale bar = 50 μm). (E, F) Western blot analysis of p-CDK9 and CDK9 protein levels in VSMCs challenged with 50 μg/ml ox-LDL for the indicated time points (n = 8; ##P < 0.01 compared to 0h).