Research Paper Volume 13, Issue 11 pp 14892—14909

Inhibition of CDK9 attenuates atherosclerosis by inhibiting inflammation and phenotypic switching of vascular smooth muscle cells

CDK9 inhibitor reduces indices of atherosclerotic lesions in ApoE-/- mice fed with HFD. (A–B) Representative en face Oil Red O staining and quantification of Oil Red O-positive lipid area in the aorta (n = 8; ###p ***p C) Photomicrographs showing representative H&E staining of atherosclerotic lesions (scale bar = 500 μm). (D) Oil Red O staining of atherosclerotic lesions in the aortic root (scale bar = 500 μm) and quantification lesions area highlighted by Oil Red O staining. (E) Representative images of Masson’s Trichome staining for collagen deposition (scale bar = 50 μm). (F) Serum levels of TG, TC, LDL and HDL (n = 8; #P ##P ###p *P **P

Figure 2. CDK9 inhibitor reduces indices of atherosclerotic lesions in ApoE-/- mice fed with HFD. (AB) Representative en face Oil Red O staining and quantification of Oil Red O-positive lipid area in the aorta (n = 8; ###p < 0.001 compared to STD, ***p < 0.001 compared to HFD;). (C) Photomicrographs showing representative H&E staining of atherosclerotic lesions (scale bar = 500 μm). (D) Oil Red O staining of atherosclerotic lesions in the aortic root (scale bar = 500 μm) and quantification lesions area highlighted by Oil Red O staining. (E) Representative images of Masson’s Trichome staining for collagen deposition (scale bar = 50 μm). (F) Serum levels of TG, TC, LDL and HDL (n = 8; #P < 0.05, ##P < 0.01 and ###p < 0.001 compared to STD; *P < 0.05, **P < 0.01 compared to HFD).