Research Paper Volume 13, Issue 9 pp 13211—13224

Circular RNA Plek promotes fibrogenic activation by regulating the miR-135b-5p/TGF-βR1 axis after spinal cord injury

CircPlek is upregulated in the spinal tissues after SCI. (A–D) Animal models and sequencing processes, hematoxylin and eosin staining of spinal cord samples. Sham group (A), 1 day (B), 3 days (C), and 7 days (D) after spinal cord injury (SCI). (E) The schematic diagram indicates the genomic loci of the Plek gene and CircPlek. (Ch11:16971464–17052381). (F) CircPlek was validated in spinal tissues using RT-PCR. CircPlek could be amplified by convergent primers from both cDNA and gDNA. Divergent primers amplified CircPlek in cDNA but not gDNA. (G) The sequencing of PCR products obtained using divergent primers for CircPlek confirmed the head-to-tail junction point. (H) The relative expression of CircPlek was measured in spinal tissues on days 3, 7, and 14 post-SCI. Data are expressed as means ± standard deviations. **p ***p

Figure 2. CircPlek is upregulated in the spinal tissues after SCI. (AD) Animal models and sequencing processes, hematoxylin and eosin staining of spinal cord samples. Sham group (A), 1 day (B), 3 days (C), and 7 days (D) after spinal cord injury (SCI). (E) The schematic diagram indicates the genomic loci of the Plek gene and CircPlek. (Ch11:16971464–17052381). (F) CircPlek was validated in spinal tissues using RT-PCR. CircPlek could be amplified by convergent primers from both cDNA and gDNA. Divergent primers amplified CircPlek in cDNA but not gDNA. (G) The sequencing of PCR products obtained using divergent primers for CircPlek confirmed the head-to-tail junction point. (H) The relative expression of CircPlek was measured in spinal tissues on days 3, 7, and 14 post-SCI. Data are expressed as means ± standard deviations. **p < 0.01, ***p < 0.001.