Research Paper Volume 13, Issue 11 pp 14924—14939

Nucleolar small molecule RNA SNORA75 promotes endometrial receptivity by regulating the function of miR-146a-3p and ZNF23

SNORA75 is upregulated in the mid-secretive phase endometrium. (A) The expression of SNORNA in proliferative (PE) and mid secretory (MSE) endometrium was analyzed by a heatmap. (B) The expression of SNORNA in the proliferative (PE) and mid secretory (MSE) endometrium was detected by real-time quantitative fluorescence PCR. (C) The expression of SNORNA in proliferative (PE) and mid secretory (MSE) endometrium was detected by real-time quantitative fluorescence PCR. (D) Real-time quantitative PCR was used to detect SNORNA expression in different stages of the endometrium. PE: proliferative phase, ESE: early secretory phase, MSE: secretory phase, LSE: late secretory phase. (E) Real-time quantitative fluorescence PCR was used to detect snoRNA expression in different stages of the endometrium. (F) Real-time quantitative PCR was used to detect the temporal expression of SNORNA in the placenta. (G) The expression of SNORA75 in EEC, ESC, Ishikawa and RL952 cells was detected by real-time quantitative fluorescence PCR. (H) The expression of SNORA75 in the nucleus and cytoplasm was detected by real-time quantitative fluorescence PCR. (I) In situ hybridization was used to detect SNORA75 expression in endometrial cells. Blue represents the nucleus, and red represents SNORA75.

Figure 1. SNORA75 is upregulated in the mid-secretive phase endometrium. (A) The expression of SNORNA in proliferative (PE) and mid secretory (MSE) endometrium was analyzed by a heatmap. (B) The expression of SNORNA in the proliferative (PE) and mid secretory (MSE) endometrium was detected by real-time quantitative fluorescence PCR. (C) The expression of SNORNA in proliferative (PE) and mid secretory (MSE) endometrium was detected by real-time quantitative fluorescence PCR. (D) Real-time quantitative PCR was used to detect SNORNA expression in different stages of the endometrium. PE: proliferative phase, ESE: early secretory phase, MSE: secretory phase, LSE: late secretory phase. (E) Real-time quantitative fluorescence PCR was used to detect snoRNA expression in different stages of the endometrium. (F) Real-time quantitative PCR was used to detect the temporal expression of SNORNA in the placenta. (G) The expression of SNORA75 in EEC, ESC, Ishikawa and RL952 cells was detected by real-time quantitative fluorescence PCR. (H) The expression of SNORA75 in the nucleus and cytoplasm was detected by real-time quantitative fluorescence PCR. (I) In situ hybridization was used to detect SNORA75 expression in endometrial cells. Blue represents the nucleus, and red represents SNORA75.