Research Paper Volume 13, Issue 11 pp 14924—14939

Nucleolar small molecule RNA SNORA75 promotes endometrial receptivity by regulating the function of miR-146a-3p and ZNF23

SNORA75 combined with miR-146a-3p. (A) MS2bs RNA pull-down was used to detect the binding of human SNORA75 and miRNA. (B) MS2bs RNA pull-down was used to detect the binding of mouse SNORA75 and miRNA. (C) Biotin-mir-146a-3p RNA pull-down was used to detect the binding of hsa-mir-146a-3p to SNORA75. (D) Biotin-miR-146a-3p RNA pull-down was used to detect the binding of mmu-mir-146a-3p to SNORA75. (E) A luciferase reporter gene was used to detect the binding of miR-146a-3p to hsa-SNORA75. (F) A luciferase reporter gene was used to detect the binding of miR-146a-3p to hsa-SNORA75. "##" indicates that, compared with the pLKO.1-vector group, P **" indicates that, compared with the pLVX-vector group, P

Figure 4. SNORA75 combined with miR-146a-3p. (A) MS2bs RNA pull-down was used to detect the binding of human SNORA75 and miRNA. (B) MS2bs RNA pull-down was used to detect the binding of mouse SNORA75 and miRNA. (C) Biotin-mir-146a-3p RNA pull-down was used to detect the binding of hsa-mir-146a-3p to SNORA75. (D) Biotin-miR-146a-3p RNA pull-down was used to detect the binding of mmu-mir-146a-3p to SNORA75. (E) A luciferase reporter gene was used to detect the binding of miR-146a-3p to hsa-SNORA75. (F) A luciferase reporter gene was used to detect the binding of miR-146a-3p to hsa-SNORA75. "##" indicates that, compared with the pLKO.1-vector group, P < 0.01. "**" indicates that, compared with the pLVX-vector group, P < 0.01.