Research Paper Volume 13, Issue 11 pp 14924—14939

Nucleolar small molecule RNA SNORA75 promotes endometrial receptivity by regulating the function of miR-146a-3p and ZNF23

SNORA75 promotes ZNF23 expression by modulating miR-146a-3p. (A) The effect of miR-146a-3p on ZNF23 mRNA expression was detected by real-time quantitative fluorescence PCR. (B) The effect of miR-146a-3p on ZNF23 protein expression was detected by Western blotting. (C) The binding of miR-146a-3p to hsa-ZNF23 was detected by the luciferase reporter assay. (D) The effect of ZNF23 overexpression on the expression of receptivity-related factors in EEC cells was detected by real-time quantitative PCR. (E) Western blotting was used to detect the effect of ZNF23 overexpression on the expression of receptivity-related factors in EEC cells. (F) The effect of ZNF23 knockdown on the expression of receptivity-related factors in EEC cells was detected by real-time quantitative fluorescence PCR. (G) Western blotting was used to detect the effect of ZNF23 knockdown on the expression of receptivity-related factors in EEC cells. (H) ZNF23 expression was detected by Western blotting. (I) ZNF23 expression was detected by Western blotting. "**" indicates P

Figure 6. SNORA75 promotes ZNF23 expression by modulating miR-146a-3p. (A) The effect of miR-146a-3p on ZNF23 mRNA expression was detected by real-time quantitative fluorescence PCR. (B) The effect of miR-146a-3p on ZNF23 protein expression was detected by Western blotting. (C) The binding of miR-146a-3p to hsa-ZNF23 was detected by the luciferase reporter assay. (D) The effect of ZNF23 overexpression on the expression of receptivity-related factors in EEC cells was detected by real-time quantitative PCR. (E) Western blotting was used to detect the effect of ZNF23 overexpression on the expression of receptivity-related factors in EEC cells. (F) The effect of ZNF23 knockdown on the expression of receptivity-related factors in EEC cells was detected by real-time quantitative fluorescence PCR. (G) Western blotting was used to detect the effect of ZNF23 knockdown on the expression of receptivity-related factors in EEC cells. (H) ZNF23 expression was detected by Western blotting. (I) ZNF23 expression was detected by Western blotting. "**" indicates P < 0.01.