Figure 8. (A) The expression levels of CTSC mRNA decreased after siRNA transfection in SHG-44 and U251 cells. (B) CCK-8 assays showed that inhibition of CTSC suppressed proliferation of SHG-44 and U251 cells. (C) Numbers and images of positive SA-b-gal staining cells in control, siRNA-NC, CTSC-inhibition cells are shown. (D) Images and histograms showing colony formation and numbers in the SHG-44 and U251 cells. (E) Diagrams showing the percentage distribution of SHG-44 and U251 cells stained with PI in the different phases of the cell cycle. (siRNA-NC, siRNA negative control, NS, p>0.05, * p < 0.05, **p<0.01, ***p<0.001, ****p<0.0001).