Figure 4. ADA alleviated microglial activation in CCH rats. (A) Representative blots of Iba1 in the different groups. β-Actin was used as loading control. (B) Quantification of the optical intensity of Iba1 in (A). (C) mRNA expression of Iba1 in the different groups. (D) Representative immunofluorescence images of Iba1 in the different groups. (E) Quantification of Iba1+ cells in (D). All values are expressed as the mean ± SEM (n = 6). **p < 0.01 vs. the sham group; ##p < 0.01 vs. the CCH group.