Research Paper Volume 13, Issue 9 pp 13264—13286

Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

Exosomal circRNA

Figure 4. Exosomal circRNA_102481 derived from EGFR-TKIs resistance cells. (A) circRNA_102481 expression levels in exosomes were almost equal to those in CCM, but, the levels of circRNA_102481 expression in EGFR-TKIs-resistant cells were significantly higher than in CCM and exosomes, #p > 0.05, *p< 0.05. (B) RT-qPCR was performed to detect the expression level of circRNA_102481 after treatment with 1 μg/ml RNase alone or combined with 0.1% Triton X-100 for 30 min, #p > 0.05, *p< 0.05 compared to the PBS control. (C) Transfection efficiency of circRNA_102481 was verified by a confocal laser scanning microscope and RT-qPCR assay. **P < 0.01 versus si-NC group cells. (D) circRNA_102481 was significantly down- regulated in exosomes of si-circRNA_ 102481 cells. *p<0.05, **p<0.01 versus si-NC group exosomes. CCM: cell culture medium.