Figure 1. SnoRD126 regulates the phosphorylation of AKT and the expression of FGFR2. (A) qRT-PCR assay for snoRD126 expression in normal human hepatocyte 7702 cells and HCC cell lines. (B) qRT-PCR assay for FGFR2 expression in snoRD126-overexpressing Huh7 cells. (C) Phosphorylation of AKT was determined by immunoblotting in snoRD126-overexpressing Huh7 cells. (D) qRT-PCR assay for snoRD126 knockdown in HepG2 cells treated with ASOs. (E) The mRNA levels of FGFR2 in snoRD126-knockdown HepG2 cells as measured by qRT-PCR assay. αS1, αS2, and αS3 are ASOs that target snoRD126. HepG2 cells were treated with 25μM ASO for 24 hours. (F) Phosphorylation of AKT was reduced in snoRD126-knockdown HepG2 cells as measured by immunoblotting. The data represent mean ± SD (n = 3). *P < 0.05, **P < 0.01. 7702 cell in (A), loading control.