Research Paper Volume 13, Issue 9 pp 13300—13317

SnoRD126 promotes the proliferation of hepatocellular carcinoma cells through transcriptional regulation of FGFR2 activation in combination with hnRNPK

SnoRD126 regulates the phosphorylation of AKT and the expression of FGFR2. (A) qRT-PCR assay for snoRD126 expression in normal human hepatocyte 7702 cells and HCC cell lines. (B) qRT-PCR assay for FGFR2 expression in snoRD126-overexpressing Huh7 cells. (C) Phosphorylation of AKT was determined by immunoblotting in snoRD126-overexpressing Huh7 cells. (D) qRT-PCR assay for snoRD126 knockdown in HepG2 cells treated with ASOs. (E) The mRNA levels of FGFR2 in snoRD126-knockdown HepG2 cells as measured by qRT-PCR assay. αS1, αS2, and αS3 are ASOs that target snoRD126. HepG2 cells were treated with 25μM ASO for 24 hours. (F) Phosphorylation of AKT was reduced in snoRD126-knockdown HepG2 cells as measured by immunoblotting. The data represent mean ± SD (n = 3). *P A), loading control.

Figure 1. SnoRD126 regulates the phosphorylation of AKT and the expression of FGFR2. (A) qRT-PCR assay for snoRD126 expression in normal human hepatocyte 7702 cells and HCC cell lines. (B) qRT-PCR assay for FGFR2 expression in snoRD126-overexpressing Huh7 cells. (C) Phosphorylation of AKT was determined by immunoblotting in snoRD126-overexpressing Huh7 cells. (D) qRT-PCR assay for snoRD126 knockdown in HepG2 cells treated with ASOs. (E) The mRNA levels of FGFR2 in snoRD126-knockdown HepG2 cells as measured by qRT-PCR assay. αS1, αS2, and αS3 are ASOs that target snoRD126. HepG2 cells were treated with 25μM ASO for 24 hours. (F) Phosphorylation of AKT was reduced in snoRD126-knockdown HepG2 cells as measured by immunoblotting. The data represent mean ± SD (n = 3). *P < 0.05, **P < 0.01. 7702 cell in (A), loading control.