Research Paper Volume 13, Issue 8 pp 10989—11009

Translational control of gene expression by eIF2 modulates proteostasis and extends lifespan

Translation-dependent changes of gene expression mediated by eIF2α phosphorylation during the pre-stationary phase of growth. (A, B) Scatter plots comparing log2 fold change in total mRNA levels and in translation efficiencies (TE) of (A) WT and (B) eIF2α.S52A cells growing exponentially (0 h) and after 6 h of growth in YES medium. (C) Scatter plot comparing log2 fold change in TE between WT and eIF2α.S52A cells growing exponentially (0 h) and after 6 h of growth in YES medium. (D) Same as in (C), but restricted to the group of genes whose translation efficiency increased in WT as well as in eIF2α.S52A cells. Dotted line indicates equal values in both strains. Data information: (A–C) The best fitting line of the data is shown. R2 (A) = 0.7605; R2 (B) = 0.6947; R2 (C) = 0.5977.

Figure 5. Translation-dependent changes of gene expression mediated by eIF2α phosphorylation during the pre-stationary phase of growth. (A, B) Scatter plots comparing log2 fold change in total mRNA levels and in translation efficiencies (TE) of (A) WT and (B) eIF2α.S52A cells growing exponentially (0 h) and after 6 h of growth in YES medium. (C) Scatter plot comparing log2 fold change in TE between WT and eIF2α.S52A cells growing exponentially (0 h) and after 6 h of growth in YES medium. (D) Same as in (C), but restricted to the group of genes whose translation efficiency increased in WT as well as in eIF2α.S52A cells. Dotted line indicates equal values in both strains. Data information: (AC) The best fitting line of the data is shown. R2 (A) = 0.7605; R2 (B) = 0.6947; R2 (C) = 0.5977.