Research Paper Volume 13, Issue 10 pp 14065—14077

TCF-3-mediated transcription of lncRNA HNF1A-AS1 targeting oncostatin M expression inhibits epithelial-mesenchymal transition via TGFβ signaling in gastroenteropancreatic neuroendocrine neoplasms

HNF1A-AS1 was down-regulated in human G-NENs tissues and GEP-NENs cells. (A) HNF1A-AS1 expression was analyzed by GeneChip assay in G-NENs tissues (n = 3) and peri-tumor tissues (>5 cm distant from cancer tissues) (n = 3). (B–C) HNF1A-AS1 was decreased in these three G-NENs tissues than in peri-tumor tissues. (D) HNF1A-AS1 expression was decreased in GEP-NENs cells than in human normal pancreatic cells HPNE as analyzed by qRT-PCR. (E) Isolation of nuclear and cytoplasmic fractions in QGP-1 and STC-1 cells suggested that HNF1A-AS1 was mainly localized in the nucleus. *p **p

Figure 1. HNF1A-AS1 was down-regulated in human G-NENs tissues and GEP-NENs cells. (A) HNF1A-AS1 expression was analyzed by GeneChip assay in G-NENs tissues (n = 3) and peri-tumor tissues (>5 cm distant from cancer tissues) (n = 3). (BC) HNF1A-AS1 was decreased in these three G-NENs tissues than in peri-tumor tissues. (D) HNF1A-AS1 expression was decreased in GEP-NENs cells than in human normal pancreatic cells HPNE as analyzed by qRT-PCR. (E) Isolation of nuclear and cytoplasmic fractions in QGP-1 and STC-1 cells suggested that HNF1A-AS1 was mainly localized in the nucleus. *p < 0.05, **p < 0.01.