Research Paper Volume 13, Issue 9 pp 12334—12358

The nuclear sirtuin SIRT6 protects the heart from developing aging-associated myocyte senescence and cardiac hypertrophy

Sirt6.Tg mice are protected from aging induced cardiac hypertrophy. (A) Heart weight to tibia length (HW/TL) ratio of 24-month-old Wild type and 24-month-old Sirt6 transgenic (Sirt6.Tg) mice. Values are mean ± SE, n = 8-10. (B) Expression levels of βMHC and ANF mRNA levels in Wild type and Sirt6.Tg mice, mean ± SE, n=6 mice. (C) Representative sections of hearts stained with Masson's trichrome to detect fibrosis (blue); scale bars, 20 μm. (D) Quantification of cardiac fibrosis in Wild type and Sirt6.Tg mice. Mean ± SE, n = 6. (E) Heart lysates of Wild type and Sirt6.Tg mice were subjected to immunoblotting using indicated antibodies. Representative blots of three different mice in each group are shown, n = 6. (Quantification of blots is given in Supplementary Figure 6A–6F).

Figure 6. Sirt6.Tg mice are protected from aging induced cardiac hypertrophy. (A) Heart weight to tibia length (HW/TL) ratio of 24-month-old Wild type and 24-month-old Sirt6 transgenic (Sirt6.Tg) mice. Values are mean ± SE, n = 8-10. (B) Expression levels of βMHC and ANF mRNA levels in Wild type and Sirt6.Tg mice, mean ± SE, n=6 mice. (C) Representative sections of hearts stained with Masson's trichrome to detect fibrosis (blue); scale bars, 20 μm. (D) Quantification of cardiac fibrosis in Wild type and Sirt6.Tg mice. Mean ± SE, n = 6. (E) Heart lysates of Wild type and Sirt6.Tg mice were subjected to immunoblotting using indicated antibodies. Representative blots of three different mice in each group are shown, n = 6. (Quantification of blots is given in Supplementary Figure 6A6F).