Research Paper Volume 13, Issue 9 pp 12334—12358

The nuclear sirtuin SIRT6 protects the heart from developing aging-associated myocyte senescence and cardiac hypertrophy

Sirt6.Tg mice display reduced cardiac aging phenotype. (A) Quantification of NAD+ contents in the heart lysate of 24-month-old WT and 24-month-old Sirt6 transgenic (Sirt6.Tg) mice. Values are mean ± SE, n = 8. (B) Relative Mitochondrial citrate synthase activity in the heart of Wild type and Sirt6.Tg mice. Values are mean ± SE, n = 5. (C) Relative mitochondrial lesions in the heart of Wild type and Sirt6.Tg mice. Values are mean ± SE, n = 5. (D) 8-Oxo-dG content in the DNA of the whole heart of Wild type and Sirt6.Tg mice. Values are mean ± SE, n = 5. (E) Relative telomere length of Wild type and Sirt6.Tg mice. Values are mean ± SE, n = 5.

Figure 7. Sirt6.Tg mice display reduced cardiac aging phenotype. (A) Quantification of NAD+ contents in the heart lysate of 24-month-old WT and 24-month-old Sirt6 transgenic (Sirt6.Tg) mice. Values are mean ± SE, n = 8. (B) Relative Mitochondrial citrate synthase activity in the heart of Wild type and Sirt6.Tg mice. Values are mean ± SE, n = 5. (C) Relative mitochondrial lesions in the heart of Wild type and Sirt6.Tg mice. Values are mean ± SE, n = 5. (D) 8-Oxo-dG content in the DNA of the whole heart of Wild type and Sirt6.Tg mice. Values are mean ± SE, n = 5. (E) Relative telomere length of Wild type and Sirt6.Tg mice. Values are mean ± SE, n = 5.