Research Paper Volume 13, Issue 9 pp 12359—12377

Intravitreal injection of anti-miRs against miR-142-3p reduces angiogenesis and microglia activation in a mouse model of laser-induced choroidal neovascularization

Overexpression of miR-142-3p in a laser-induced CNV mouse model increases both vascular and inflammatory phenotypes. (A) OCT measurement presented as (b-c)/c ratios where b is the CNV lesion thickness and c is the adjacent choroid thickness. b and c were measured just prior to sacrifice (n = 24-26 per experimental group). (B) Flat-mounted choroids showing vascular (in green) and microglia (in red) density and corresponding quantification. Scale bar = 250 μm (n = 11-13 per experimental group). All results are presented as mean +- SEM. Mann Whitney test (* = p ≤ 0.05).

Figure 3. Overexpression of miR-142-3p in a laser-induced CNV mouse model increases both vascular and inflammatory phenotypes. (A) OCT measurement presented as (b-c)/c ratios where b is the CNV lesion thickness and c is the adjacent choroid thickness. b and c were measured just prior to sacrifice (n = 24-26 per experimental group). (B) Flat-mounted choroids showing vascular (in green) and microglia (in red) density and corresponding quantification. Scale bar = 250 μm (n = 11-13 per experimental group). All results are presented as mean +- SEM. Mann Whitney test (* = p ≤ 0.05).