Research Paper Volume 13, Issue 9 pp 12359—12377

Intravitreal injection of anti-miRs against miR-142-3p reduces angiogenesis and microglia activation in a mouse model of laser-induced choroidal neovascularization

MiR-142-3p regulates the activation of human microglia cells under pro-inflammatory conditions. (A–C) Transfections of miR-142-3p inhibitor and mimic lead to decreased and increased miR-142-3p level, respectively (A). Transfection of miR-142-3p mimic decreased BCLAF1 levels, a previously identified target of miR-142-3p, both at the transcript level (B) and protein level (C). (D, E) Effects on CD68 level (D) or VEGF-A (E) in HMC3 transfected with miR-142-3p inhibitor or mimic and relative controls. Only miR-142-3p mimic was able to induce CD68 et VEGF-A production. (F) HMC3 stimulated with either IFNᵞ or LPS for 24 hours overexpress miR-142-3p as long with CD68. All qRT-PCR results are presented as mean +- SEM. Mann Whitney test. (* = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001; **** = p ≤ 0.0001; ns = not significant).

Figure 5. MiR-142-3p regulates the activation of human microglia cells under pro-inflammatory conditions. (AC) Transfections of miR-142-3p inhibitor and mimic lead to decreased and increased miR-142-3p level, respectively (A). Transfection of miR-142-3p mimic decreased BCLAF1 levels, a previously identified target of miR-142-3p, both at the transcript level (B) and protein level (C). (D, E) Effects on CD68 level (D) or VEGF-A (E) in HMC3 transfected with miR-142-3p inhibitor or mimic and relative controls. Only miR-142-3p mimic was able to induce CD68 et VEGF-A production. (F) HMC3 stimulated with either IFN or LPS for 24 hours overexpress miR-142-3p as long with CD68. All qRT-PCR results are presented as mean +- SEM. Mann Whitney test. (* = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001; **** = p ≤ 0.0001; ns = not significant).