Figure 2. CircITCH promotes ECM degradation of degenerative NP cells. (A–E) The NP cells were infected with lentiviral plasmids carrying circITCH shRNA or corresponding control shRNA or transfected with the pcDNA3.1 or the pcDNA3.1-circITCH overexpression vector. (A–D) The mRNA expression of collagen II (A), aggrecan (B), MMP13 (C), and ADAMTS4 (D) was measured by qPCR in the cells. (E) The protein expression of collagen II, aggrecan, MMP13, ADAMTS4, and β-actin was tested by Western blot analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: * P < 0.05.