Research Paper Volume 13, Issue 10 pp 14185—14197

Circular RNA ITCH promotes extracellular matrix degradation via activating Wnt/β-catenin signaling in intervertebral disc degeneration

MiR-17-5p inhibits ECM degradation in the degenerative NP cells. (A–F) The NP cells were treated with the miR-17-5p mimic or control mimic. (A) The expression levels of miR-17-5p were tested by qPCR in the cells. (B–E) The mRNA expression of collagen II (B), aggrecan (C), MMP13 (D), and ADAMTS4 (E) was measured by qPCR in the cells. (F) The protein expression of collagen II, aggrecan, MMP13, ADAMTS4, and β-actin was determined by Western blot analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: * P P

Figure 4. MiR-17-5p inhibits ECM degradation in the degenerative NP cells. (AF) The NP cells were treated with the miR-17-5p mimic or control mimic. (A) The expression levels of miR-17-5p were tested by qPCR in the cells. (BE) The mRNA expression of collagen II (B), aggrecan (C), MMP13 (D), and ADAMTS4 (E) was measured by qPCR in the cells. (F) The protein expression of collagen II, aggrecan, MMP13, ADAMTS4, and β-actin was determined by Western blot analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: * P < 0.05, ** P < 0.01.