Research Paper Volume 13, Issue 10 pp 14198—14218

LncRNA LOC146880 promotes esophageal squamous cell carcinoma progression via miR-328-5p/FSCN1/MAPK axis

LOC146880 expression is upregulated in ESCC tissues and cells. (A) The expression levels of LOC146880 in 21 pairs of ESCC and adjacent normal esophageal tissues. (B) Kaplan-Meier survival curve analysis shows overall survival of ESCC patients with high- and low-expression levels of LOC146880 (n = 81). (C) QRT-PCR analysis shows LOC146880 expression levels in human esophageal epithelial cells and ESCC cell lines (EC109, TE-1, Kyse30, Kyse70, Kyse150, and Kyse410). (D) FISH analysis shows LOC146880 expression in ESCC and normal esophageal tissues. (E) Bioinformatics analysis (<a href="https://genome.ucsc.edu/" target="

Figure 1. LOC146880 expression is upregulated in ESCC tissues and cells. (A) The expression levels of LOC146880 in 21 pairs of ESCC and adjacent normal esophageal tissues. (B) Kaplan-Meier survival curve analysis shows overall survival of ESCC patients with high- and low-expression levels of LOC146880 (n = 81). (C) QRT-PCR analysis shows LOC146880 expression levels in human esophageal epithelial cells and ESCC cell lines (EC109, TE-1, Kyse30, Kyse70, Kyse150, and Kyse410). (D) FISH analysis shows LOC146880 expression in ESCC and normal esophageal tissues. (E) Bioinformatics analysis (https://genome.ucsc.edu/) shows H3K27ac levels in the promoter region of LOC146880. (F) ChIP assay analysis with anti- H3K27ac antibody shows H3K27ac levels in the LOC146880 promoter of HEEC, Kyse30, and TE-1 cells. (G) ChIP assay analysis with anti-CBP antibody shows CBP protein levels in the LOC146880 promoter of HEEC, Kyse30, and TE-1 cells. (H) Western blot analysis shows CBP protein levels in control and CBP-silenced Kyse30, and TE-1 cells. (I) ChIP assay analysis shows H3K27ac levels in the LOC146880 promoter of control and CBP-silenced Kyse30, and TE-1 cells. (J) QRT-PCR analysis shows LOC146880 expression levels in control and CBP-silenced Kyse30 and TE-1 cells. *P < 0.05, **P < 0.01, ***P < 0.001.