Research Paper Volume 13, Issue 10 pp 14198—14218

LncRNA LOC146880 promotes esophageal squamous cell carcinoma progression via miR-328-5p/FSCN1/MAPK axis

LOC146880 silencing reduces in vivo ESCC growth and progression. (A) Representative images show nude mice xenografted with sh-NC-transfected Kyse30 cells (upper, n = 10) and sh-LOC146880-transfected Kyse30 cells (lower, n = 10). (B) Representative images show ESCC-derived tumor xenografts from nude mice injected with sh-NC-transfected Kyse30 cells (upper) and sh-LOC146880-transfected Kyse30 cells (lower). (C) Tumor volumes of sh-NC and sh-LOC146880 groups of ESCC xenograft tumors in nude mice. (D) Tumor mass of sh-NC and sh-LOC146880 groups of ESCC xenograft tumors in nude mice. (E) Representative images and quantitative analysis show the number of metastatic tumor nodules in the liver of nude mice belonging to sh-NC and sh-LOC146880 groups. (F–G) QRT-PCR analysis of (F) LOC146880 and (G) miR-328-5p expression levels in xenograft tumors from sh-NC and sh-LOC146880 groups. (H) Western blot analysis shows FSCN1 protein levels in xenograft tumors from sh-NC and sh-LOC146880 groups. (I) Immunohistochemical staining and Tunel Staining show3 expression levels of FSCN1, Ki-67 and apoptotic cells in xenograft tumor sections from sh-NC and sh-LOC146880 groups.

Figure 7. LOC146880 silencing reduces in vivo ESCC growth and progression. (A) Representative images show nude mice xenografted with sh-NC-transfected Kyse30 cells (upper, n = 10) and sh-LOC146880-transfected Kyse30 cells (lower, n = 10). (B) Representative images show ESCC-derived tumor xenografts from nude mice injected with sh-NC-transfected Kyse30 cells (upper) and sh-LOC146880-transfected Kyse30 cells (lower). (C) Tumor volumes of sh-NC and sh-LOC146880 groups of ESCC xenograft tumors in nude mice. (D) Tumor mass of sh-NC and sh-LOC146880 groups of ESCC xenograft tumors in nude mice. (E) Representative images and quantitative analysis show the number of metastatic tumor nodules in the liver of nude mice belonging to sh-NC and sh-LOC146880 groups. (FG) QRT-PCR analysis of (F) LOC146880 and (G) miR-328-5p expression levels in xenograft tumors from sh-NC and sh-LOC146880 groups. (H) Western blot analysis shows FSCN1 protein levels in xenograft tumors from sh-NC and sh-LOC146880 groups. (I) Immunohistochemical staining and Tunel Staining show3 expression levels of FSCN1, Ki-67 and apoptotic cells in xenograft tumor sections from sh-NC and sh-LOC146880 groups.