Research Paper Volume 13, Issue 10 pp 14219—14233

LncRNA AK020546 protects against cardiac ischemia–reperfusion injury by sponging miR-350-3p

lncRNA AK020546 inhibited I/R-induced oxidative stress and apoptosis in vitro. (A) qPCR verified the lncRNA AK020546-overexpressing efficiency of adenovirus particles in H9c2 cardiomyocytes (n = 5). (B) TUNEL staining was used to detect the apoptosis of H9c2 cardiomyocytes (n = 5). (C, D) Flow cytometry and Annexin V/propidium iodide (PI) staining were carried out to detect H9c2 cardiomyocyte apoptosis (n = 5). (E–G) The levels of oxidative markers, such as LDH, MDA, and ROS, were detected using commercial kits (n = 5). *p #p 2O2 + Ad-nc or control group.

Figure 2. lncRNA AK020546 inhibited I/R-induced oxidative stress and apoptosis in vitro. (A) qPCR verified the lncRNA AK020546-overexpressing efficiency of adenovirus particles in H9c2 cardiomyocytes (n = 5). (B) TUNEL staining was used to detect the apoptosis of H9c2 cardiomyocytes (n = 5). (C, D) Flow cytometry and Annexin V/propidium iodide (PI) staining were carried out to detect H9c2 cardiomyocyte apoptosis (n = 5). (EG) The levels of oxidative markers, such as LDH, MDA, and ROS, were detected using commercial kits (n = 5). *p < 0.05 versus Ad-nc or control group, #p < 0.05 vs H2O2 + Ad-nc or control group.