Research Paper Volume 13, Issue 10 pp 14219—14233

LncRNA AK020546 protects against cardiac ischemia–reperfusion injury by sponging miR-350-3p


Figure 3. LncRNA AK020546 inhibited I/R injury-induced oxidative stress and apoptosis in vivo. (A) qPCR confirmed the lncRNA AK020546-overexpressing efficiency of adenovirus particles in the myocardium in comparison with the control group (n = 8). (BD) The levels of oxidative markers, such as LDH, MDA, and ROS, in the myocardium were detected using commercial kits (n = 8). (E) TTC staining was performed to evaluate the infarct area of the heart (n = 8). (F) TUNEL staining and α-smooth muscle actin (SMA) staining were performed to detect the apoptosis of specific H9c2 cardiomyocytes (n = 8). *p < 0.05 vs Ad-nc or sham group, #p < 0.05 vs I/R + Ad-nc or control group.