Research Paper Volume 13, Issue 10 pp 14219—14233

LncRNA AK020546 protects against cardiac ischemia–reperfusion injury by sponging miR-350-3p

LncRNA AK020546 sponged miR-350-3p in H9c2 cardiomyocytes. (A) The potential targeting region predicted by bioinformatics analysis. (B) Luciferase assay was performed to verify whether miR-350-3p targeted AK020546 in H9c2 cardiomyocytes (n = 5). (C) RNA pull-down assay confirmed the interaction between AK020546 and miR-350-3p (n = 5). (D) qPCR was used to detect the expression of AK020546 in different groups (n = 5). (E) Pearson’s analysis was performed to investigate the correlation between AK020546 and miR-350-3p (n = 5). *p #p

Figure 5. LncRNA AK020546 sponged miR-350-3p in H9c2 cardiomyocytes. (A) The potential targeting region predicted by bioinformatics analysis. (B) Luciferase assay was performed to verify whether miR-350-3p targeted AK020546 in H9c2 cardiomyocytes (n = 5). (C) RNA pull-down assay confirmed the interaction between AK020546 and miR-350-3p (n = 5). (D) qPCR was used to detect the expression of AK020546 in different groups (n = 5). (E) Pearson’s analysis was performed to investigate the correlation between AK020546 and miR-350-3p (n = 5). *p < 0.05 versus miR-nc or biotin-nc, #p < 0.05 vs inhibitor nc group.