Research Paper Volume 13, Issue 10 pp 14258—14276

LncRNA LOC100129620 promotes osteosarcoma progression through regulating CDK6 expression, tumor angiogenesis, and macrophage polarization

LncRNA LOC100129620 regulates osteosarcoma-induced angiogenesis. (A) Cell scratch assay to detect the migration ability of HUVECs treated with cell culture supernatant from U2OS cells with LOC100129620 overexpression or knockdown. (B) Cell scratch assay to detect the migration ability of HUVECs treated with cell culture supernatant isolated from MG63 cells with LOC100129620 overexpression or knockdown. (C) Transwell assay to detect the invasion ability of HUVECs treated with cell culture supernatant isolated from U2OS cells with LOC100129620 overexpression or knockdown. (D) Quantitative analysis of the Transwell assay results of HUVECs treated with cell culture supernatant isolated from U2OS cells with LOC100129620 overexpression or knockdown. (E) Transwell assay to detect the invasion ability of HUVECs treated with cell culture supernatant isolated from MG63 cells with LOC100129620 overexpression or knockdown. (F) Quantitative analysis of the Transwell assay results of HUVECs treated with cell culture supernatant isolated from MG63 cells with LOC100129620 overexpression or knockdown. Statistical analysis was conducted using Student’s t-test. (G) Immunofluorescence analysis to detect the expression of VEGF in xenograft tumor. (H) Immunofluorescence analysis to detect the expression of CD34 in xenograft tumor. Values are means ± SD. **P ##P

Figure 7. LncRNA LOC100129620 regulates osteosarcoma-induced angiogenesis. (A) Cell scratch assay to detect the migration ability of HUVECs treated with cell culture supernatant from U2OS cells with LOC100129620 overexpression or knockdown. (B) Cell scratch assay to detect the migration ability of HUVECs treated with cell culture supernatant isolated from MG63 cells with LOC100129620 overexpression or knockdown. (C) Transwell assay to detect the invasion ability of HUVECs treated with cell culture supernatant isolated from U2OS cells with LOC100129620 overexpression or knockdown. (D) Quantitative analysis of the Transwell assay results of HUVECs treated with cell culture supernatant isolated from U2OS cells with LOC100129620 overexpression or knockdown. (E) Transwell assay to detect the invasion ability of HUVECs treated with cell culture supernatant isolated from MG63 cells with LOC100129620 overexpression or knockdown. (F) Quantitative analysis of the Transwell assay results of HUVECs treated with cell culture supernatant isolated from MG63 cells with LOC100129620 overexpression or knockdown. Statistical analysis was conducted using Student’s t-test. (G) Immunofluorescence analysis to detect the expression of VEGF in xenograft tumor. (H) Immunofluorescence analysis to detect the expression of CD34 in xenograft tumor. Values are means ± SD. **P < 0.01, pLVX-Vector group compared with pLVX-LOC100129620 group; ##P < 0.01, pLKO.1-Vector group compared with pLKO.1-sh1 and pLKO.1-sh2 groups.