Research Paper Volume 13, Issue 10 pp 14277—14288

Silencing UCHL3 enhances radio-sensitivity of non-small cell lung cancer cells by inhibiting DNA repair

Expression of UCHL3 is up-regulated in non-small cell lung cancer tissues and cell lines. (A, B) Western blot analysis (A) and real-time PCR (B) analysis of UCHL3 expression in 12 paired tumor (T) tissues and adjacent non-tumor (N) tissues. (C, D) Western blot analysis (C) and real-time PCR (D) analysis of UCHL3 expression in normal lung epithelial cell lines BEAS-2B and non-small cell lung cancer (NSCLC) cell lines A649, H1975, H1299, H460, H520. (E, F) Kaplan-Meier analysis of OS (E), PFS (F) in NSCLC patients. Data was obtained from Kaplan-Meier Plotter website. OS, overall survival. PFS, progression-free survival. β-actin was used as a loading control. **P

Figure 1. Expression of UCHL3 is up-regulated in non-small cell lung cancer tissues and cell lines. (A, B) Western blot analysis (A) and real-time PCR (B) analysis of UCHL3 expression in 12 paired tumor (T) tissues and adjacent non-tumor (N) tissues. (C, D) Western blot analysis (C) and real-time PCR (D) analysis of UCHL3 expression in normal lung epithelial cell lines BEAS-2B and non-small cell lung cancer (NSCLC) cell lines A649, H1975, H1299, H460, H520. (E, F) Kaplan-Meier analysis of OS (E), PFS (F) in NSCLC patients. Data was obtained from Kaplan-Meier Plotter website. OS, overall survival. PFS, progression-free survival. β-actin was used as a loading control. **P < 0.01.