Research Paper Volume 13, Issue 10 pp 14277—14288

Silencing UCHL3 enhances radio-sensitivity of non-small cell lung cancer cells by inhibiting DNA repair

UCHL3 knockdown enhanced radio-sensitivity of non-small cell lung cancer cells in vitro. (A, B) Western blot analysis (A) and real-time PCR (B) analysis of UCHL3 expression in A549 control cells and cells exposed to ionizing radiation (IR). (C, D) Western blot analysis (C) and real-time PCR (D) analysis of UCHL3 expression in H460 control cells and cells exposed to IR. (E, F) Western blot analysis (E) and real-time PCR (F) analysis of UCHL3 expression in A549 cells transfected with sh-NC or sh-UCHL3. (G, H) Western blot analysis (G) and real-time PCR (H) analysis of UCHL3 expression in H460 cells transfected with sh-NC or sh-UCHL3. (I) Colony formation assay of A549 cells and H460 cells were transfected with sh-NC or sh-UCHL3 and received the corresponding radiation does (0, 2, 4, 6, 8 Gy). (J) Relative growth rate in A549 cells and H460 cells has shown. The relative growth rates are reported as the mean ± s.d. from three independent experiments. β-actin was used as a loading control. **P

Figure 2. UCHL3 knockdown enhanced radio-sensitivity of non-small cell lung cancer cells in vitro. (A, B) Western blot analysis (A) and real-time PCR (B) analysis of UCHL3 expression in A549 control cells and cells exposed to ionizing radiation (IR). (C, D) Western blot analysis (C) and real-time PCR (D) analysis of UCHL3 expression in H460 control cells and cells exposed to IR. (E, F) Western blot analysis (E) and real-time PCR (F) analysis of UCHL3 expression in A549 cells transfected with sh-NC or sh-UCHL3. (G, H) Western blot analysis (G) and real-time PCR (H) analysis of UCHL3 expression in H460 cells transfected with sh-NC or sh-UCHL3. (I) Colony formation assay of A549 cells and H460 cells were transfected with sh-NC or sh-UCHL3 and received the corresponding radiation does (0, 2, 4, 6, 8 Gy). (J) Relative growth rate in A549 cells and H460 cells has shown. The relative growth rates are reported as the mean ± s.d. from three independent experiments. β-actin was used as a loading control. **P < 0.01.