Research Paper Volume 13, Issue 10 pp 14277—14288

Silencing UCHL3 enhances radio-sensitivity of non-small cell lung cancer cells by inhibiting DNA repair

UCHL3 knockdown enhanced ionizing radiation-induced DNA damage. (A) γ-H2AX staining in A549 cells transfected with sh-NC and sh-UCHL3 after exposure to ionizing radiation (IR). (B) Western blot analysis of γ-H2AX, p-Chk2, Chk2 after A549 cells treated with indicated treatment. (C) γ-H2AX staining in H460 cells transfected with sh-NC and sh-UCHL3 after exposure to IR. (D) Western blot analysis of γ-H2AX, p-Chk2, Chk2 after H460 cells treated with indicated treatment. β-actin was used as a loading control. **P

Figure 4. UCHL3 knockdown enhanced ionizing radiation-induced DNA damage. (A) γ-H2AX staining in A549 cells transfected with sh-NC and sh-UCHL3 after exposure to ionizing radiation (IR). (B) Western blot analysis of γ-H2AX, p-Chk2, Chk2 after A549 cells treated with indicated treatment. (C) γ-H2AX staining in H460 cells transfected with sh-NC and sh-UCHL3 after exposure to IR. (D) Western blot analysis of γ-H2AX, p-Chk2, Chk2 after H460 cells treated with indicated treatment. β-actin was used as a loading control. **P < 0.01.