Research Paper Volume 13, Issue 10 pp 14304—14321

Accumulation of CD45RO+CD8+ T cells is a diagnostic and prognostic biomarker for clear cell renal cell carcinoma

CD8+ T cells in peripheral blood and tissues of ccRCC patients. We analyzed clinical and bioinformatics data of CD8+ T cells. (A) Proportion of CD8+ T cells (CD3+CD4-CD8+) in CD3+ cells of peripheral blood from healthy people (n = 350) and ccRCC patients (n = 274). (B) Proportion of CD8+ T cells in peripheral CD3+ cells of different grades of ccRCC (grade I: n = 198; grade II: n = 28; grade III: n = 32; grade IV: n = 16). (C) CD8a transcript level of ccRCC tissues (n = 533) and normal renal tissues (n = 72) according to TCGA database. (D) CD8a transcript level of different grades ccRCC (normal: n = 72; grade I: n = 14; grade II: n = 229; grade III: n = 206; grade IV: n = 76). (E) According to TCIA database, proportion of immune cells in different grades of ccRCC tissues. (F) Cumulative survival time of patients with high level of CD8+ T cells and low level of CD8+ T cells. (G) Representative IHC image of different stage of ccRCC tissues. (H) Relative quantification of staining of ccRCC tissues. Means ± SEM of experiment performed in triplicates are shown. *P **p ****p

Figure 1. CD8+ T cells in peripheral blood and tissues of ccRCC patients. We analyzed clinical and bioinformatics data of CD8+ T cells. (A) Proportion of CD8+ T cells (CD3+CD4-CD8+) in CD3+ cells of peripheral blood from healthy people (n = 350) and ccRCC patients (n = 274). (B) Proportion of CD8+ T cells in peripheral CD3+ cells of different grades of ccRCC (grade I: n = 198; grade II: n = 28; grade III: n = 32; grade IV: n = 16). (C) CD8a transcript level of ccRCC tissues (n = 533) and normal renal tissues (n = 72) according to TCGA database. (D) CD8a transcript level of different grades ccRCC (normal: n = 72; grade I: n = 14; grade II: n = 229; grade III: n = 206; grade IV: n = 76). (E) According to TCIA database, proportion of immune cells in different grades of ccRCC tissues. (F) Cumulative survival time of patients with high level of CD8+ T cells and low level of CD8+ T cells. (G) Representative IHC image of different stage of ccRCC tissues. (H) Relative quantification of staining of ccRCC tissues. Means ± SEM of experiment performed in triplicates are shown. *P < 0.05; **p < 0.01; ****p < 0.001; NS, not significant.