Research Paper Volume 13, Issue 10 pp 14304—14321

Accumulation of CD45RO+CD8+ T cells is a diagnostic and prognostic biomarker for clear cell renal cell carcinoma

MAPK signaling promotes proliferation and inhibits apoptosis of CD45RO+CD8+T cells. CD45RO+CD8+T cells and CD45RO-CD8+T cells were sorted by flow cytometry. (A) Cell viability was tested by CCK8 assay at 0, 24 and 48 hours. (B) Ki67 fluorescence intensity of CD45RO+CD8+T cells and CD45RO-CD8+T cells were tested by flow cytometry. (C) Proliferation rate of CD45RO+CD8+T cells and CD45RO-CD8+T cells were tested by flow cytometry according to changes of CFSE fluorescence intensity. (D) Apoptosis status of CD45RO-CD8+T cells and CD45RO+CD8+T cells tested by flow cytometry with PI and Annexin V stanning (right) and apoptosis proportion rates were counted (right). (E) Clustering of differential genes between CD45RO-CD8+T cells and CD45RO+CD8+T cells. (F) Quantitative analysis of p38/MAPK and p-p38/MAPK of CD45RO+CD8+T cells and CD45RO-CD8+T cells. (G) p38/MAPK and p-p38/MAPK of CD45RO+CD8+T cells after dehydrocorydaline chloride treated for 48 hours. (H) Cell viability was tested by CCK8 assay at 0, 24 and 48 hours after dehydrocorydaline chloride treated. (I) Ki67 fluorescence intensity of CD45RO+CD8+T cells were tested by flow cytometry after treated by dehydrocorydaline chloride treated for 48 hours. (J) Proliferation rate of CD45RO+CD8+T cells were tested by flow cytometry according to changes of CFSE fluorescence intensity. (K) Proportion of apoptosis CD45RO+CD8+T cells after dehydrocorydaline chloride treated for 48 hours.

Figure 5. MAPK signaling promotes proliferation and inhibits apoptosis of CD45RO+CD8+T cells. CD45RO+CD8+T cells and CD45RO-CD8+T cells were sorted by flow cytometry. (A) Cell viability was tested by CCK8 assay at 0, 24 and 48 hours. (B) Ki67 fluorescence intensity of CD45RO+CD8+T cells and CD45RO-CD8+T cells were tested by flow cytometry. (C) Proliferation rate of CD45RO+CD8+T cells and CD45RO-CD8+T cells were tested by flow cytometry according to changes of CFSE fluorescence intensity. (D) Apoptosis status of CD45RO-CD8+T cells and CD45RO+CD8+T cells tested by flow cytometry with PI and Annexin V stanning (right) and apoptosis proportion rates were counted (right). (E) Clustering of differential genes between CD45RO-CD8+T cells and CD45RO+CD8+T cells. (F) Quantitative analysis of p38/MAPK and p-p38/MAPK of CD45RO+CD8+T cells and CD45RO-CD8+T cells. (G) p38/MAPK and p-p38/MAPK of CD45RO+CD8+T cells after dehydrocorydaline chloride treated for 48 hours. (H) Cell viability was tested by CCK8 assay at 0, 24 and 48 hours after dehydrocorydaline chloride treated. (I) Ki67 fluorescence intensity of CD45RO+CD8+T cells were tested by flow cytometry after treated by dehydrocorydaline chloride treated for 48 hours. (J) Proliferation rate of CD45RO+CD8+T cells were tested by flow cytometry according to changes of CFSE fluorescence intensity. (K) Proportion of apoptosis CD45RO+CD8+T cells after dehydrocorydaline chloride treated for 48 hours.