Figure 3. TNF-α was targeted by miR-454-3p in 293T cells. (A) The predicted binding site between TNF-α and miR-454-3p. (B) The predicted miR-454-3p binding site (TNF-α-wt) and its matched mutant site (TNF-α-mut). (C) Luciferase activity detection. (D) RT-qPCR analyses measured relative miR-454-3p and TNF-α expression levels. (E) RT-qPCR analyses measured TNF-α expression levels. (F) Elisa analyses measured TNF-α expression levels. (G) Scanning electron microscope results of bone slices (500x). (H) Volume of resorption/pit (x103 um3). **P < 0.01, ***P < 0.001.