Research Paper Volume 13, Issue 10 pp 14416—14432

miR-485 inhibits histone deacetylase HDAC5, HIF1α and PFKFB3 expression to alleviate epilepsy in cellular and rodent models

miR-485 suppresses apoptosis, oxidative stress, and inflammation through HDAC5/HIF1α/PFKFB3 upregulation in epilepsy hippocampal neurons. (A) Protein expression of HDAC5, HIF1α, and PFKFB3. (B) Cell apoptosis determined by flow cytometry. (C) SOD levels. (D) GSH-Px levels. (E) MDA levels. (F) DNA 8-OHdG levels. (G) IL-1β, TNF-α, and IL-6 levels. * p p

Figure 4. miR-485 suppresses apoptosis, oxidative stress, and inflammation through HDAC5/HIF1α/PFKFB3 upregulation in epilepsy hippocampal neurons. (A) Protein expression of HDAC5, HIF1α, and PFKFB3. (B) Cell apoptosis determined by flow cytometry. (C) SOD levels. (D) GSH-Px levels. (E) MDA levels. (F) DNA 8-OHdG levels. (G) IL-1β, TNF-α, and IL-6 levels. * p < 0.05 vs. epilepsy cells treated with mimic-NC + oe-NC; # p < 0.05 vs. epilepsy cells treated with miR-485 mimic + oe-NC. Data were expressed as mean ± standard deviation. Data between two groups were compared independent sample t-test. Data among multiple groups were compared by one-way analysis of variance and post-hoc Tukey’s test. Experiments were repeated three times.