Research Paper Volume 13, Issue 10 pp 14416—14432

miR-485 inhibits histone deacetylase HDAC5, HIF1α and PFKFB3 expression to alleviate epilepsy in cellular and rodent models

miR-485 inhibits epilepsy through HDAC5/HIF1α/PFKFB3 axis in vivo. (A) Protein expression of HDAC5, HIF1α, and PFKFB3 in the brain tissues. (B) Cell apoptosis determined by TUNEL assay (400 ×). (C) SOD levels. (D) GSH-Px levels. (E) MDA levels. (F) DNA 8-OHdG levels; (G) IL-1β, TNF-α, IL-6 levels. * p p

Figure 5. miR-485 inhibits epilepsy through HDAC5/HIF1α/PFKFB3 axis in vivo. (A) Protein expression of HDAC5, HIF1α, and PFKFB3 in the brain tissues. (B) Cell apoptosis determined by TUNEL assay (400 ×). (C) SOD levels. (D) GSH-Px levels. (E) MDA levels. (F) DNA 8-OHdG levels; (G) IL-1β, TNF-α, IL-6 levels. * p < 0.05 vs. epileptic mice treated with agomir-NC + oe-NC; # p < 0.05 vs. epileptic mice treated with miR-485 agomir + oe-NC; n = 10. Data were expressed as mean ± standard deviation. Data among multiple groups were compared by one-way analysis of variance and post-hoc Tukey’s test.