Research Paper Volume 13, Issue 10 pp 14433—14455

Chronic alcohol exposure promotes HCC stemness and metastasis through β-catenin/miR-22-3p/TET2 axis

Effects of miR-22-3p and TET2 on stemness and metastasis of HCC cells. (A) Tumorspheres formation ability of HCC cells in negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. (B) The tumorspheres were quantified. *PC, D) Population of EPCAM-positive HCC cells in negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. *PE, F) Population of CD133- positive HCC cells in negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. *PG) Representative image showing the migration of HCC cells in control, negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. (H) The migrated cells were quantified as described in the Materials and Methods. *PI) Representative image showing the invasion of HCC cells in negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. (J) The invaded cells were quantified as described in the Materials and Methods. *P

Figure 6. Effects of miR-22-3p and TET2 on stemness and metastasis of HCC cells. (A) Tumorspheres formation ability of HCC cells in negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. (B) The tumorspheres were quantified. *P<0.05. (C, D) Population of EPCAM-positive HCC cells in negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. *P<0.05. (E, F) Population of CD133- positive HCC cells in negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. *P<0.05. (G) Representative image showing the migration of HCC cells in control, negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. (H) The migrated cells were quantified as described in the Materials and Methods. *P< 0.05, n= 3. (I) Representative image showing the invasion of HCC cells in negative-control, miR-22-3p inhibitor, TET2 KO and miR-22-3p inhibitor/TET2 KO groups. (J) The invaded cells were quantified as described in the Materials and Methods. *P< 0.05, n= 3.