Research Paper Volume 13, Issue 11 pp 15013—15031

ERp44/CG9911 promotes fat storage in Drosophila adipocytes by regulating ER Ca2+ homeostasis

CG9911 modulates intracellular calcium homeostasis. (A) Fluorescence changes of w1118 and CG9911 mutant fat bodies which were treated with 10μM inomycin. Scale bar = 50 μm. (B) The dynamic changes of fluorescence intensity(ΔF/F0) in the signal fat cell of w1118 and CG9911 mutant files when stimulated with 10μM inomycin. (C) The difference of mean F/F0 between w1118 and CG9911 mutant fat cells when treated with 10μM inomycin. Data are presented as the means ± s.e.m; ** pD) The comparation of the mean data for time from basal to peak (tmax), half time raise (t1/2 on), and decay (t1/2 off) between w1118 and CG9911 mutant fat cells when treated with 10μM inomycin. Data are presented as the means ± s.e.m; ** pE) Ca2+ imaging of w1118 and CG9911 mutant fat bodies when treated with 10 μM TG. (F) The dynamic changes of fluorescence intensity(ΔF/F0) in the signal fat cell of w1118 and CG9911 mutant files when stimulated with 10μM TG. (G) The difference of mean data of F/F0 between w1118 and CG9911 mutant fat bodies when treated with 10 μM TG. Data are presented as the means ± s.e.m; *** pH) The comparation of the mean data for time from basal to peak (tmax), half time raise (t1/2 on), and decay (t1/2 off) between w1118 and CG9911 mutant fat cells when treated with 10μM TG. Data are presented as the means ± s.e.m; *pI) The quiescent Ca2+ level in the signal fat cell of w1118 and CG9911 mutant files.

Figure 5. CG9911 modulates intracellular calcium homeostasis. (A) Fluorescence changes of w1118 and CG9911 mutant fat bodies which were treated with 10μM inomycin. Scale bar = 50 μm. (B) The dynamic changes of fluorescence intensity(ΔF/F0) in the signal fat cell of w1118 and CG9911 mutant files when stimulated with 10μM inomycin. (C) The difference of mean F/F0 between w1118 and CG9911 mutant fat cells when treated with 10μM inomycin. Data are presented as the means ± s.e.m; ** p<0.01. (D) The comparation of the mean data for time from basal to peak (tmax), half time raise (t1/2 on), and decay (t1/2 off) between w1118 and CG9911 mutant fat cells when treated with 10μM inomycin. Data are presented as the means ± s.e.m; ** p<0.01, *** p<0.001. (E) Ca2+ imaging of w1118 and CG9911 mutant fat bodies when treated with 10 μM TG. (F) The dynamic changes of fluorescence intensity(ΔF/F0) in the signal fat cell of w1118 and CG9911 mutant files when stimulated with 10μM TG. (G) The difference of mean data of F/F0 between w1118 and CG9911 mutant fat bodies when treated with 10 μM TG. Data are presented as the means ± s.e.m; *** p<0.001. (H) The comparation of the mean data for time from basal to peak (tmax), half time raise (t1/2 on), and decay (t1/2 off) between w1118 and CG9911 mutant fat cells when treated with 10μM TG. Data are presented as the means ± s.e.m; *p<0.05. (I) The quiescent Ca2+ level in the signal fat cell of w1118 and CG9911 mutant files.